Month: February 2022

Abstract This chapter describes the use of N-terminal glutathione S-transferase (GST)-tagged Recombinant gene fusion proteins as a method for high-level protein expression and inducible purification from bacterial cell lysates. The protein is expressed in a pGEX vector, with the GST residue located at the N-terminus followed by the target protein. The use of GST as … Read more

Some species show intersex variations in their rate of meiotic activity. recombination, where recombination is usually suppressed in the heterogametic sex. Although there is no heteromorphic sex chromosomes have been detected in the zebrafish (Danio rerio) recombinant, genetic analysis has indicated a lower frequency of recombination in males relative to females. Our study of the … Read more

Abstract For years, the use of polyhistidine tags (His-tags) has been a staple in the isolation of recombinant proteins in immobilized metal affinity chromatography experiments. Its use has been highly beneficial in increasing the protein purity of crude cell lysates. For some recombinant proteins, one consequence of the addition of the His tag is that … Read more