Some species show intersex variations in their rate of meiotic activity. recombination, where recombination is usually suppressed in the heterogametic sex. Although there is no heteromorphic sex chromosomes have been detected in the zebrafish (Danio rerio) recombinant, genetic analysis has indicated a lower frequency of recombination in males relative to females. Our study of the meiotic recombination patterns in female zebrafish indicates that adult females have only a few meiotic oocytes that are found in groups in the ventral area of the ovarian surface.
We use antibody staining of human mutL homologous protein 1 (MLH1) to mark the sites of putative chiasmata to search for a physical basis for the recombination pattern and its relationship frequency in both sexes. We report that foci of MLH1 are found mainly in distal regions of synaptonemal complexes (SC) in males, but tend to be more evenly distributed in females. Our cytological analysis shows a proportion of MLH1 foci per chromosome in males versus females of 1:1.55. this low level of recombination in males is in general agreement with previously published results of linkage map analysis.
However, the similar proportion of MLH1 foci per unit length of SC in both sexes demonstrates a correlation between SC length and frequency of recombination instead of a mechanism that suppresses recombination in males. Therefore, chiasmal interference seems to provide a similar expression in males and females according to the situation in humans, where oocytes with longer SC show a higher level of recombination that is not a consequence of more closely crosses spaced along with the SCs. Heritage (2008) 100, 489–495; doi:10.1038/SJ.day.6801091;
posted online March 5, 2008
Keywords: zebrafish; mitosis; recombination; chiasmata; MLH1; synaptonemal complex
Purity: greater than 90% as determined by SDS-PAGE.
Destination names: sod1
Uniprot No.: O73872
Research area: Signal transduction
Alternative names: sod1; because superoxide dismutase [Cu-Zn]; EC 1.15.1.1
Species: Danio rerio (Zebrafish) (Brachydanio rerio)
Source: E.coli
Expression Region: 1-154aa
Mole Weight: 37.1 kDa
Protein Length: Total length
Tag information: N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Form: Liquid or Lyophilized Powder
Note: We will preferably ship the format we have in stock, however, if you have any special requirements for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the dosage form is liquid, the default storage buffer is Tris/PBS based buffer, 5%-50% glycerol.
Note:
If you have any special requirement for glycerol content, please remark it when you order. If the administration form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged before opening to bring the contents to the bottom. Reconstitute protein in sterile deionized water at a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and an aliquot for long-term storage at -20°C/-80°C. Our final default glycerol concentration is 50%. Customers could use it for reference.
Storage Conditions
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Shelf Life
Shelf life is related to many factors, including storage status, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.
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Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.