Radiation resistance is a serious explanation for lung most cancers therapy failure. Armadillo (ARM) superfamily proteins take part in numerous basic mobile processes; nevertheless, whether or not ARM proteins regulate radiation resistance just isn’t totally understood. Right here, we used an unbiased CRISPR/Cas9 library display screen and recognized plakophilin 2 (PKP2), a member of the ARM superfamily of proteins, as a important driver of radiation resistance in lung most cancers.
The PKP2 stage was considerably larger after radiotherapy than earlier than radiotherapy, and excessive PKP2 expression after radiotherapy predicted poor general survival (OS) and postprogression survival (PPS). Mechanistically, mass spectrometry evaluation recognized that PKP2 was methylated on the arginine web site and interacted with protein arginine methyltransferase 1 (PRMT1). Methylation of PKP2 by PRMT1 stabilized β-catenin by recruiting USP7, additional inducing LIG4, a key DNA ligase in nonhomologous end-joining (NHEJ) restore.
Concomitantly, PKP2-induced radioresistance relied on facilitating LIG4-mediated NHEJ restore in lung most cancers. Extra strikingly, after publicity to irradiation, therapy with the PRMT1 inhibitor C-7280948 abolished PKP2-induced radioresistance, and C-7280948 is a possible radiosensitizer in lung most cancers. In abstract, our outcomes display that focusing on the PRMT1/PKP2/β-catenin/LIG4 pathway is an efficient method to beat radiation resistance in lung most cancers. Utilizing CRISPR/Cas9 dual-guide ribonucleic proteins, we present selective and combinatorial mutagenesis of 17 genes at 28 goal websites with efficiencies exceeding 85% in grownup F0 ‘crispants’.
Environment friendly CRISPR/Cas9 mutagenesis for neurobehavioral screening in grownup zebrafish
Grownup zebrafish are more and more used to interrogate mechanisms of illness growth and tissue regeneration. But, the prospect of large-scale genetics in grownup zebrafish has historically confronted a bunch of organic and technical challenges, together with inaccessibility of grownup tissues to high-throughput phenotyping and the spatial and technical calls for of grownup husbandry. Right here, we describe an experimental pipeline that mixes high-efficiency CRISPR/Cas9 mutagenesis with useful phenotypic screening to determine genes required for spinal wire restore in grownup zebrafish.
We discover that capillary electrophoresis is a dependable methodology to measure indel frequencies. Utilizing a quantifiable behavioral assay, we determine seven single- or duplicate-gene crispants with lowered useful restoration after spinal wire damage. To rule out off-target results, we generate germline mutations that recapitulate the crispant regeneration phenotypes. This examine supplies a platform that mixes high-efficiency somatic mutagenesis with a useful phenotypic readout to carry out medium- to large-scale genetic research in grownup zebrafish.
An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides allow strong and speedy COVID-19 testing
Intensive testing is important to interrupt the transmission of SARS-CoV-2, which causes the continued COVID-19 pandemic. Right here, we current a CRISPR-based diagnostic assay that’s strong to viral genome mutations and temperature, produces outcomes quick, will be utilized immediately on nasopharyngeal (NP) specimens with out RNA purification, and incorporates a human inside management throughout the similar response. Particularly, we present that using an engineered AsCas12a enzyme permits detection of wildtype and mutated SARS-CoV-2 and permits us to carry out the detection step with loop-mediated isothermal amplification (LAMP) at 60-65 °C.
We additionally discover that using hybrid DNA-RNA guides will increase the speed of response, enabling our take a look at to be accomplished inside 30 minutes. Using scientific samples from 72 sufferers with COVID-19 an infection and 57 wholesome people, we display that our take a look at displays a specificity and constructive predictive worth of 100% with a sensitivity of 50 and 1000 copies per response (or 2 and 40 copies per microliter) for purified RNA samples and unpurified NP specimens respectively.
Enhancement of phycoerythrin productiveness in Porphyridium purpureum utilizing the clustered recurrently interspaced quick palindromic repeats/CRISPR-associated protein 9 ribonucleoprotein system
Genetic manipulation of the Porphyridium sp. could improve the manufacturing of phycoerythrin. Since phycobiliproteins seize and switch power to each photosystems (PS I and PS II), it was hypothesized that the gene mutation concerned will increase phycoerythrin synthesis. The gene encoding chlorophyll synthase (CHS1) was chosen as chlorophyll synthase performs an vital position in photosynthesis, mediating the ultimate means of chlorophyll synthesis.
Clustered recurrently interspaced quick palindromic repeats/CRISPR-associated protein 9 ribonucleoprotein (CRISPR/Cas9 RNP) supply system was used to generate the chlorophyll synthase loss-of-function mutants (Δchs1). Unbiased Δchs1 confirmed no variations within the progress and manufacturing of sulfated polysaccharide in comparison with management. Phycoerythrin contents of the 2 unbiased mutants considerably elevated no matter mild supply. This examine supplies a novel applicability for the CRISPR/Cas9 RNP methodology in crimson microalgae towards a bio-product of curiosity. The obtained mutants may function potential producers of phycoerythrin if Porphyridium is chosen as a pure supply.
A extra environment friendly CRISPR-Cas12a variant derived from Lachnospiraceae bacterium MA2020
CRISPR effector proteins introduce double-stranded breaks into the mammalian genome, facilitating gene enhancing by non-homologous end-joining or homology-directed restore. Not like the extra generally studied Cas9, the CRISPR effector protein Cas12a/Cpf1 acknowledges a T-rich protospacer adjoining motif (PAM) and may course of its personal CRISPR RNA (crRNA) array, simplifying using a number of information RNAs. We noticed that the Cas12a ortholog of Lachnospiraceae bacterium MA2020 (Lb2Cas12a) edited mammalian genes with efficiencies akin to these of AsCas12a and LbCas12a. In comparison with these well-characterized Cas12a orthologs, Lb2Cas12a is smaller and acknowledges a slim set of PAM TTTV. We launched two mutations into Lb2Cas12a
 Rabbit Glutathione peroxidase ELISA kit |
|
E04G0369-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rabbit Glutathione peroxidase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
) Rabbit IgG SABC Kit (Peroxidase) |
|
abx090693-1Kit |
Abbexa |
1 Kit |
EUR 314 |
|
|
 Rabbit Type I Collagen Detection Kit |
|
6016 |
Chondrex |
1 kit |
EUR 673.5 |
|
Description: Rabbit Type I Collagen Detection Kit |
 ELISA kit) Rabbit Eosinophil peroxidase(EPX) ELISA kit |
|
E04E0383-192T |
BlueGene |
192 tests |
EUR 1270 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rabbit Eosinophil peroxidase(EPX) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Eosinophil peroxidase(EPX) ELISA kit |
|
E04E0383-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rabbit Eosinophil peroxidase(EPX) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Eosinophil peroxidase(EPX) ELISA kit |
|
E04E0383-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rabbit Eosinophil peroxidase(EPX) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 Rabbit Glutathione Peroxidase 1 ELISA kit |
|
E04G0234-192T |
BlueGene |
192 tests |
EUR 1270 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Rabbit Glutathione Peroxidase 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Glutathione Peroxidase 1 ELISA kit |
|
E04G0234-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Rabbit Glutathione Peroxidase 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Glutathione Peroxidase 1 ELISA kit |
|
E04G0234-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Rabbit Glutathione Peroxidase 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 ELISA Kit) Rabbit Thyroid Peroxidase (TPO) ELISA Kit |
|
abx362288-96tests |
Abbexa |
96 tests |
EUR 825 |
|
|
 ELISA Kit) Rabbit Glutathione Peroxidase (GPX) ELISA Kit |
|
abx363178-96tests |
Abbexa |
96 tests |
EUR 825 |
|
|
 Rabbit Glutathione peroxidase 1, GPX1 ELISA Kit |
|
ELA-E0295Rb |
Lifescience Market |
96 Tests |
EUR 928 |
 Rabbit Anti Thyroid peroxidase antibody ELISA kit |
|
E04T0531-192T |
BlueGene |
192 tests |
EUR 1270 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rabbit Anti Thyroid peroxidase antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Anti Thyroid peroxidase antibody ELISA kit |
|
E04T0531-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rabbit Anti Thyroid peroxidase antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Anti Thyroid peroxidase antibody ELISA kit |
|
E04T0531-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rabbit Anti Thyroid peroxidase antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 ELISA Kit) Rabbit Glutathione Peroxidase 1 (GPX1) ELISA Kit |
|
abx576504-96tests |
Abbexa |
96 tests |
EUR 825 |
|
|
 GenomeCoV19 Detection Kit |
|
G628.v2 |
ABM |
100 Rxns |
EUR 320 |
|
Description: The most affordable qPCR Detection Kit for SARS-CoV-2 on the market.abm’s GenomeCoV19 Detection Kit is a real-time reverse transcription-polymerase chain reaction (RT-qPCR) test intended for the qualitative detection of RNA from SARS-CoV-2 in human nasopharyngeal and oropharyngeal swab specimens from individuals suspected of COVID-19 by their healthcare provider. Our GenomeCoV19 Detection Kit is the most affordable qPCR detection kit on the market at only $1.77 USD/test (limited time pricing for US customers only).This kit is widely used in Europe under the CE-IVD certification and is listed by U.S. Food and Drug Administration (FDA) for distribution in the USA, under Section IV.C. |
 Senescence Detection Kit |
|
55R-1370 |
Fitzgerald |
250 tests |
EUR 712 |
|
Description: Senescence Detection Kit for use in the research laboratory |
 Formaldehyde Detection Kit |
|
K001-F1 |
Arbor Assays |
2x96 well plate |
EUR 425 |
Senescence Detection Kit |
|
K320-250 |
Biovision |
|
EUR 468 |
 Autophagy Detection Kit |
|
abx299014-50tests |
Abbexa |
50 tests |
EUR 523 |
|
|
Autophagy Detection Kit |
|
abx299015-200tests |
Abbexa |
200 tests |
EUR 871 |
|
|
 HMGB1 Detection Kit |
|
6010 |
Chondrex |
1 kit |
EUR 753.25 |
|
Description: HMGB1 Detection Kit |
 Ubiquitin Detection Kit |
|
SKT-131-20 |
Stressmarq |
20 assays |
EUR 495 |
|
|
|
Description: Purification Detection kit used to capture, detect, identify and characterise ubiquitinated proteins and free chains from samples.
in Cell Lysates, Tissue samples from all species |
 Glutathione Detection Kit |
|
SKT-202-96 |
Stressmarq |
1 plate of 96 wells |
EUR 428 |
|
|
|
Description: Direct Fluorometric detection assay to measure the total GSH content in Whole Blood, Serum, EDTA Plasma, Heparin Plasma, Erythrocytes, Urine, Cell Lysates, Tissue samples from all species |
 Apoptosis Detection Kit |
|
ANXVKB-100T |
ImmunoStep |
100 test |
EUR 464.7 |
Apoptosis Detection Kit |
|
ANXVKCFB-100T |
ImmunoStep |
100 test |
EUR 412.7 |
Apoptosis Detection Kit |
|
ANXVKCFB7-100T |
ImmunoStep |
100 test |
EUR 412.7 |
Apoptosis Detection Kit |
|
ANXVKDY-100T |
ImmunoStep |
100 test |
EUR 389.3 |
Apoptosis Detection Kit |
|
ANXVKF-100T |
ImmunoStep |
100 test |
EUR 329.5 |
Apoptosis Detection Kit |
|
ANXVKF7-100T |
ImmunoStep |
100 test |
EUR 329.5 |
Apoptosis Detection Kit |
|
ANXVKPE-100T |
ImmunoStep |
100 test |
EUR 346.4 |
Senescence Detection Kit |
|
K2030-250 |
ApexBio |
250 Staining |
EUR 460 |
 Frit Kit |
|
FRIT-KIT |
Next Advance |
1each |
EUR 124 |
|
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
 Peroxidase Assay Kit |
|
Z5030052 |
Biochain |
100 assays |
EUR 622 |
|
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Peroxidase Assay Kit |
|
abx298828-100Assays |
Abbexa |
100 Assays |
EUR 425 |
|
|
 with goat anti-rabbit HRP secondary antibody) ExoAb Antibody Kit (CD9, CD63, CD81, Hsp70 antibodies, rabbit anti-human) with goat anti-rabbit HRP secondary antibody |
|
EXOAB-KIT-1 |
SBI |
25 ul each |
EUR 627 |
|
|
 Column Packing Kit |
|
PACK-KIT |
Next Advance |
1pack |
EUR 1035 |
|
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
 DB Detection KIT - rabbit/mouse dual, HRP/DAB |
|
DB-DETKIT-100 |
DB Biotech |
100 ml |
EUR 1401 |
DB Detection KIT - rabbit/mouse dual, HRP/DAB |
|
DB-DETKIT-50 |
DB Biotech |
50 ml |
EUR 777 |
DB Detection KIT - rabbit/mouse dual, HRP/DAB |
|
DBDETKIT-100 |
DB Biotech |
100 ml |
EUR 1401 |
DB Detection KIT - rabbit/mouse dual, HRP/DAB |
|
DBDETKIT-50 |
DB Biotech |
50 ml |
EUR 777 |
) Super Sensitive IHC Detection System Kit (Mouse / Rabbit) |
|
abx097195-1Kit |
Abbexa |
1 Kit |
EUR 356 |
|
|
 ELISA kit) Rabbit Epididymal secretory glutathione peroxidase(GPX5) ELISA kit |
|
E04E0389-192T |
BlueGene |
192 tests |
EUR 1270 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Rabbit Epididymal secretory glutathione peroxidase(GPX5) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Epididymal secretory glutathione peroxidase(GPX5) ELISA kit |
|
E04E0389-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Rabbit Epididymal secretory glutathione peroxidase(GPX5) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Epididymal secretory glutathione peroxidase(GPX5) ELISA kit |
|
E04E0389-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Rabbit Epididymal secretory glutathione peroxidase(GPX5) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 ELISA Kit) Rabbit Thyroid Peroxidase Antibody (TPO-Ab) ELISA Kit |
|
abx362677-96tests |
Abbexa |
96 tests |
EUR 825 |
|
|
 Protein) Rabbit Glutathione Peroxidase 1 (GPX1) Protein |
|
20-abx653588 |
Abbexa |
-
EUR 648.00
-
EUR 272.00
-
EUR 1998.00
-
EUR 773.00
-
EUR 467.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
|
|
 Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit |
|
CAS400A-KIT |
SBI |
1 kit (10 rxn) |
EUR 1110 |
|
|
 Mycoplasma PCR Detection Kit |
|
K1476-100 |
Biovision |
100 Rxns |
EUR 298 |
 MitoCapture Apoptosis Detection Kit |
|
55R-1305 |
Fitzgerald |
25 assays |
EUR 277 |
|
Description: Apoptosis Kit for detection of apoptosis in the research laboratory |
 Thiol Fluorescent Detection Kit |
|
K005-F1 |
Arbor Assays |
1x96 well plate |
EUR 318 |
 Glutathione Fluorescent Detection Kit |
|
K006-F1 |
Arbor Assays |
1x96 well plate |
EUR 354 |
 DNA Methylation Detection Kit |
|
K5082100 |
Biochain |
1 kit |
EUR 344 |
) Senescence Detection Kit (Fluorometric) |
|
K991-100 |
Biovision |
|
EUR 718 |
 PCR-Salmonella Detection Kit |
|
K1447-96 |
Biovision |
|
EUR 620 |
 PCR-STEC Detection Kit |
|
K1452-96 |
Biovision |
|
EUR 784 |
 PCR-Campylobacter Detection Kit |
|
K1453-96 |
Biovision |
|
EUR 784 |
 Residual SDS Detection Kit |
|
BSP055 |
Bio Basic |
100Assays |
EUR 83.06 |
|
|
 Luciferase Mycoplasma Detection Kit |
|
20-abx298016 |
Abbexa |
-
EUR 1010.00
-
EUR 300.00
-
EUR 425.00
|
|
|
|
 PCR Mycoplasma Detection Kit |
|
abx298017-100rxns |
Abbexa |
100 rxns |
EUR 314 |
|
|
 Protein A Detection Kit |
|
abx090813-1Kit |
Abbexa |
1 Kit |
EUR 1581 |
|
|
Luciferase Mycoplasma Detection Kit |
|
20-abx098882 |
Abbexa |
-
EUR 1233.00
-
EUR 523.00
-
EUR 648.00
|
|
|
|
PCR Mycoplasma Detection Kit |
|
abx098883-100rxns |
Abbexa |
100 rxns |
EUR 537 |
|
|
 Mouse Albumin Detection Kit |
|
3012 |
Chondrex |
1 kit |
EUR 483.55 |
|
Description: Mouse Albumin Detection Kit |
 Rat Albumin Detection Kit |
|
3020 |
Chondrex |
1 kit |
EUR 483.55 |
|
Description: Rat Albumin Detection Kit |
 Ubiquitin Interact Detection Kit |
|
SKT-132-20 |
Stressmarq |
20 assays |
EUR 464 |
|
|
|
Description: Purification Detection kit used to capture, detect, identify and characterise isolated ubiquitin binding proteins by Western blotting or proteomic analysis in samples.
in Cell Lysates, Tissue samples from all species |
 Urine Creatinine Detection Kit |
|
SKT-200-192 |
Stressmarq |
2 plates of 96 wells |
EUR 342 |
|
|
|
Description: Direct Detection kit used for quantitative measuring creatinine in Urine samples from Human, Monkey, Dog, Rat |
 Nitric Oxide Detection Kit |
|
SKT-212-96 |
Stressmarq |
2 plates of 96 wells |
EUR 342 |
|
|
|
Description: Indirect Colorimetric assay used for quantitative measuring Nitrate and Nitrite present in a variety of samples in Serum, Plasma, Urine, Saliva, Water, Buffer, Cell Lysates, Tissue Culture Media samples from all species |
 Hydrogen Peroxide Detection Kit |
|
SKT-216-192 |
Stressmarq |
2 plates of 96 wells |
EUR 441 |
|
|
|
Description: Direct Colorimetric assay used for quantitative measuring H2O2 in a variety of samples in Urine, Buffer, Tissue Culture Media samples from all species |
 Creatinine Serum Detection Kit |
|
SKT-217-192 |
Stressmarq |
2 plates of 96 wells |
EUR 342 |
|
|
|
Description: Direct Colorimetric assay used to measure creatinine in Serum, Plasma samples from Human, Mouse, Rabbit, Rat, Sheep |
 MitoStep + Apoptosis Detection Kit |
|
KMAF-100T |
ImmunoStep |
100 test |
EUR 420.5 |
) Mycoplasma Detection Kit (OKSB00010) |
|
OKSB00010 |
Aviva Systems Biology |
50 Tests |
EUR 382 |
|
Description: Description of target: ;Species reactivity: ;Application: PCR;Assay info: ;Sensitivity: |
 CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
|
CASLV100PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
|
 CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
|
CASLV105PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
|
 MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
|
CASLV120PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
|
 MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
|
CASLV125PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
|
 Glutathione Peroxidase Assay Kit |
|
55R-1522 |
Fitzgerald |
100 assays |
EUR 706 |
|
Description: Assay Kit for detection of Glutathione Reductase in the research laboratory |
 Thyroid peroxidase ELISA kit |
|
55R-ORG503 |
Fitzgerald |
96 wells |
EUR 372 |
|
Description: ELISA kit for the detection of Thyroid peroxidase in the research laboratory |
 QuantiChrom Peroxidase Assay Kit |
|
D2PD-100 |
BioAssay Systems |
100 |
EUR 351 |
|
Description: Quantitative determination of peroxidase activity by colorimetric (570nm) or fluorometric (530nm/590nm) methods. Procedure: 20 min. Kit size: 100 tests. Detection limit: 0.8 U/L. Shelf life: 3 months. Shipping: ambient temp; storage: 4, -20°C. |
Glutathione Peroxidase Assay Kit |
|
20-abx294002 |
Abbexa |
|
|
|
|
 Ascorbate Peroxidase Assay Kit |
|
abx298818-100Assays |
Abbexa |
100 Assays |
EUR 566 |
|
|
 Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
|
CAS700A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
|
 Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
|
CAS720A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
|
 Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
|
CAS740A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
|
) T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) |
|
CAS510A-KIT |
SBI |
1 Kit |
EUR 805 |
|
|
 ELISA kit) Rabbit Phospholipid hydroperoxide glutathione peroxidase, mitochondrial(GPX4) ELISA kit |
|
E04P0808-192T |
BlueGene |
192 tests |
EUR 1270 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rabbit Phospholipid hydroperoxide glutathione peroxidase, mitochondrial(GPX4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Phospholipid hydroperoxide glutathione peroxidase, mitochondrial(GPX4) ELISA kit |
|
E04P0808-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rabbit Phospholipid hydroperoxide glutathione peroxidase, mitochondrial(GPX4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Phospholipid hydroperoxide glutathione peroxidase, mitochondrial(GPX4) ELISA kit |
|
E04P0808-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rabbit Phospholipid hydroperoxide glutathione peroxidase, mitochondrial(GPX4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|
CASLV200PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
|
-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|
CASLV205PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
|
-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|
CASLV220PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
|
-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|
CASLV225PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
|
 Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
|
CAS750A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
|
 Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
|
CAS770A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
|
 Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
|
CAS790A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
|
 and Fast ligase (2.5ul)]) Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)] |
|
CAS9LIG-KIT |
SBI |
1 Kit |
EUR 153 |
|
|
 DB Detection system rabbit/mouse dual - HRP |
|
DB-DET-SYS |
DB Biotech |
100 ml |
EUR 1064 |
DB Detection system rabbit/mouse dual - HRP |
|
DBDETSYS |
DB Biotech |
100 ml |
EUR 1064 |
 Anti-Rabbit EZ-HRP™ Polymer Detection |
|
SF40006 |
Neuromics |
15 ml |
EUR 226 |
 ExtrAvidin Peroxidase |
|
abx082602-1ml |
Abbexa |
1 ml |
EUR 1038 |
|
|
 Mouse Rabbit mAb) Thyroid Peroxidase/TPO (9G3) Mouse Rabbit mAb |
|
A18609-100ul |
Abclonal |
100 ul |
EUR 384 |
Thyroid Peroxidase/TPO (9G3) Mouse Rabbit mAb |
|
A18609-200ul |
Abclonal |
200 ul |
Ask for price |
Thyroid Peroxidase/TPO (9G3) Mouse Rabbit mAb |
|
A18609-20ul |
Abclonal |
20 ul |
Ask for price |
Thyroid Peroxidase/TPO (9G3) Mouse Rabbit mAb |
|
A18609-50ul |
Abclonal |
50 ul |
EUR 265 |
) Goat anti-Rabbit IgG SecondaryAntibody (peroxidase conjugated) |
|
E0L3012-1 |
EnoGene |
500ul |
EUR 128 |
Goat anti-Rabbit IgG SecondaryAntibody (peroxidase conjugated) |
|
E0L3012-2 |
EnoGene |
1000ul |
EUR 180 |
) PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
|
PIN320A-KIT |
SBI |
1 Kit |
EUR 4941 |
|
|
) PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
|
PIN340iPS-KIT |
SBI |
1 Kit |
EUR 4941 |
|
|
) Super Sensitive FITC IF Detection System Kit (Goat Anti-Rabbit) |
|
abx097199-1Kit |
Abbexa |
1 Kit |
EUR 272 |
|
|
) Super Sensitive FITC IF Detection System Kit (Rabbit Anti-Sheep) |
|
abx097201-1Kit |
Abbexa |
1 Kit |
EUR 272 |
|
|
) Super Sensitive TRITC IF Detection System Kit (Goat Anti-Rabbit) |
|
abx097203-1Kit |
Abbexa |
1 Kit |
EUR 272 |
|
|
) Super Sensitive TRITC IF Detection System Kit (Rabbit Anti-Sheep) |
|
abx097205-1Kit |
Abbexa |
1 Kit |
EUR 272 |
|
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, Rz >3.0) Peroxidase, horseradish (Horseradish peroxidase - HRP), Rz >3.0 |
|
PB0691 |
Bio Basic |
100mg |
EUR 141.35 |
|
|
 CRISPR Genomic Cleavage Detection Kit |
|
G932 |
ABM |
25 Reactions |
EUR 205 |
 TUNEL FITC Apoptosis Detection Kit |
|
A111-01 |
Vazyme |
20 rxn |
EUR 283 |
TUNEL FITC Apoptosis Detection Kit |
|
A111-02 |
Vazyme |
50 rxn |
EUR 409 |
TUNEL FITC Apoptosis Detection Kit |
|
A111-03 |
Vazyme |
100 rxn |
EUR 539 |
 TUNEL BrightGreen Apoptosis Detection Kit |
|
A112-01 |
Vazyme |
20 rxn |
EUR 306 |
TUNEL BrightGreen Apoptosis Detection Kit |
|
A112-02 |
Vazyme |
50 rxn |
EUR 456 |
TUNEL BrightGreen Apoptosis Detection Kit |
|
A112-03 |
Vazyme |
100 rxn |
EUR 655 |
 TUNEL BrightRed Apoptosis Detection Kit |
|
A113-01 |
Vazyme |
20 rxn |
EUR 306 |
TUNEL BrightRed Apoptosis Detection Kit |
|
A113-02 |
Vazyme |
50 rxn |
EUR 456 |
TUNEL BrightRed Apoptosis Detection Kit |
|
A113-03 |
Vazyme |
100 rxn |
EUR 655 |
) Cholesterol Detection Kit (cell-based) |
|
K587-100 |
Biovision |
|
EUR 294 |
) Copper Detection Assay Kit (Fluorometric) |
|
K899-100 |
Biovision |
|
EUR 457 |
CRISPR Genomic Cleavage Detection Kit |
|
K1250-25 |
Biovision |
|
EUR 403 |
 PCR-Salmonella-Listeria Detection Kit |
|
K1448-96 |
Biovision |
|
EUR 784 |
 PCR-Listeria monocytogenes Detection Kit |
|
K1449-96 |
Biovision |
|
EUR 648 |
 PCR-Legionella spp Detection Kit |
|
K1450-96 |
Biovision |
|
EUR 648 |
 Platelet Associated Immunoglobulin Detection Kit |
|
abx290001-25assays |
Abbexa |
25 assays |
EUR 968 |
|
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Platelet Associated Immunoglobulin Detection Kit |
|
abx290001-50Assays |
Abbexa |
50 Assays |
EUR 1191 |
|
|
Q571Okay and C1003Y, that elevated its cleavage effectivity for a spread of goal sequences past these of the generally used Cas12a orthologs AsCas12a and LbCas12a. Along with the canonical TTTV PAM, this variant, Lb2-KY, additionally effectively cleaved goal areas with CTTN PAMs. Lastly, we demonstrated that Lb2-KY ribonucleoprotein (RNP) complexes edited two hemoglobin goal areas helpful for correcting frequent types of sickle-cell anemia extra effectively than business AsCas12a RNP complexes. Thus, Lb2-KY has distinctive properties helpful for modifying a spread of clinically related targets within the human genome.
