Hairpin-Spacer crRNA-Enhanced CRISPR/Cas13a System Promotes the Specificity of Single Nucleotide Polymorphism (SNP) Identification

Hairpin-Spacer crRNA-Enhanced CRISPR/Cas13a System Promotes the Specificity of Single Nucleotide Polymorphism (SNP) Identification

The Cas13a system has nice potential in RNA interference and molecular diagnostic fields. Nevertheless, missing tips for crRNA design hinders sensible purposes of the Cas13a system in RNA enhancing and single nucleotide polymorphism identification. This research posits that crRNAs with hairpin spacers enhance the specificity of CRISPR/Cas13a system (termed hs-CRISPR). Gibbs free power evaluation means … Read more